Canon 350D (Rebel XT) with 17-85 IS Lens @ 40mm (~60mm). ISO 400, 1/13 @ F6.3 hand held. Here I would have selected ISO 400 as a starting point as it is quite dark.. 1/13 second shutter speed allowed me to hand hold the camera because I was using an image stabilised lens. At a focal lenght of ~ 60mm, minimum hand holding speed would be 1/60 second (more on that when we get to shutter speeds, next). Going to ISO 1600 (1/60 @ F6.3) would have allowed me to shoot with similar results to above with a lot more noise. Ideallly in these situations a tripod was needed. Then I can have as much depth of field as I like - provided the subject doesn't move due to long exposure times. The F6.3 aperture only just provides adequate depth of field. This pic is pushing the limits of hand held natural light photography.